BWA-MEM2 alignment skill for mapping sequencing reads to reference genomes with optimized parameter selection

ClinVar database query skill for clinical variant interpretation and pathogenicity lookup

DeepVariant deep learning variant calling skill for high-accuracy SNV and indel detection

DESeq2 differential expression analysis skill with normalization, statistical modeling, and visualization

Sequencing quality control skill for assessing read quality, adapter contamination, and sequence composition

Gene fusion detection skill for oncology applications with multiple caller integration

GA4GH standards compliance skill for data sharing and interoperability

GATK best practices skill for germline and somatic variant calling with joint genotyping

Gene Set Enrichment Analysis skill for functional annotation and pathway interpretation

HIPAA compliance validation skill for genomic data handling and audit

Human Phenotype Ontology skill for phenotype-driven gene prioritization

HUMAnN functional profiling skill for metagenomic pathway analysis

Kraken2 taxonomic classification skill for rapid metagenomic read assignment

MaxQuant mass spectrometry skill for protein identification and quantification

MEGAHIT metagenomic assembly skill for reconstructing genomes from short reads

MetaPhlAn metagenomic profiling skill for species-level community composition

Nextflow workflow management skill for reproducible bioinformatics pipelines

Perseus statistical analysis skill for proteomics data analysis and visualization

PharmGKB pharmacogenomics annotation skill for drug-gene interaction assessment

Phylogenetic analysis skill for constructing evolutionary trees and assessing relationships

PyMOL molecular visualization skill for structure rendering and analysis

QIIME2 microbiome analysis skill for 16S rRNA profiling and diversity analysis

RDKit chemoinformatics skill for molecular property calculation and compound library management

Salmon pseudo-alignment skill for fast and accurate transcript quantification

Seurat single-cell analysis skill for clustering, annotation, and trajectory analysis

Singularity container management skill for HPC-compatible containerized execution

STAR alignment skill for splice-aware RNA-seq read mapping with comprehensive QC metrics

STRING protein interaction network skill for functional enrichment and network visualization

Structural variant detection skill for identifying CNVs, inversions, translocations, and complex rearrangements

Tumor mutation burden and microsatellite instability calculation skill for immunotherapy biomarkers

UCSC Genome Browser query skill for genome annotation retrieval and track data access

Variant Effect Predictor skill for comprehensive variant annotation with clinical database integration

Adverse event monitoring and reporting skill for MDR/MEDWATCH compliance

AI/ML medical device validation skill implementing FDA's GMLP principles

Biocompatibility test selection and protocol recommendation skill based on device categorization

Bioreactor culture protocol development skill for tissue construct maturation

Systematic literature review skill for clinical evaluation supporting regulatory submissions

Clinical study design skill for medical device trials including IDE studies and post-market studies

Medical device cybersecurity risk assessment skill per FDA premarket and postmarket guidance

Design Failure Mode and Effects Analysis (DFMEA) skill for systematic design risk identification

Design review planning and execution skill for structured design phase gate reviews per 21 CFR 820.30

DICOM conformance testing and integration skill for medical imaging systems

EU MDR General Safety and Performance Requirements (GSPR) mapping and compliance documentation skill

E&L study design and data analysis skill for chemical characterization of medical devices

Fatigue life prediction skill for implants and load-bearing devices using validated approaches

Automated 510(k) premarket notification preparation for generating submission packages, predicate device comparisons, and substantial equivalence arguments

Finite element mesh generation skill optimized for biomedical geometries including implants, anatomical structures, and tissue models

Medical device software lifecycle management skill implementing IEC 62304 requirements

Medical image processing algorithm validation skill for segmentation, detection, and analysis algorithms

Medical device standards compliance verification skill for ISO 13485, ISO 14971, IEC 62304, IEC 60601, and related standards